A recent study has revealed that sanitization of toothbrushes with mouthwash can effectively reduce the number of live microorganisms adhered to the filaments of toothbrushes.
Toothbrushes are colonized by microorganisms, implying a risk of infection. By reducing this microbial colonization, the risk of disease transmission can be reduced significantly. Therefore, the purpose of this study was to evaluate the antiseptic activity of a mouthwash containing 0.05% chlorhexidine and 0.05% cetylpyridinium chloride on toothbrushes.
A total of twelve toothbrushes used three times/day for 14 days by orally and systemically healthy people were randomly split into two groups, and their heads were immersed for 2 h in PBS (control) or Perio·Aid Active Control (treatment). The microorganisms were recovered, and their number was calculated by culture, quantitative PCR, and viability PCR. Statistical differences were first assessed with a two-way mixed ANOVA and subsequently with Student's t-test.
The results showed no statistical differences in the total number of cells for the treatment and control group but a significantly low number of live cells in the treatment group than in the control group.
It is to be noted that such a decrease in the bacterial load could include bacteria from the oral cavity, from the environment, and from nearby toothbrushes since the quantification was not limited to any bacterial taxon.
1. Gerard Àlvarez, Agnès Soler-Ollé, Sergio Isabal, Rubén León, Vanessa Blanc: Bacterial decontamination of toothbrushes by immersion in a mouthwash containing 0.05% chlorhexidine and 0.05% cetylpyridinium chloride: A randomized controlled trial.